BeNa Culture Collection
|Culture medium||Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.|
|Growth conditions||36 ℃;18-24h; Aerobic;|
|Growth characteristics||G-bacilli, lysine decarboxylase positive, ornithine decarboxylase, arginine dihydrolase negative, urease positive, phenylalanine deaminase negative, take-pour two test positive, indole negative, no H2S production, no power, fermentation of lactose, mannitol, inositol, sorbitol, calendolol, no fermentation of maleose. Facultative anaerobic, optimum pH7.4 ~ 7.6.|
|Storage conditions||2-8 ℃|
|morphology||Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Light yellow Protuberance: Middle convex Surface: Bright and Smooth Texture: Wet|
|application||Research, quality control, CTFA (American Cosmetic Flavor Association) recommends bacteria for cosmetic microorganism challenge|
|Sharing mode||Public welfare sharing|
Storage conditions: 2~8 ℃
Product format: freeze dried,200ul
Validity period: Freeze-dried tube for 6 years
Biosafety level: 2, handle in biosafety cabinet
Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:37°C, aerobic, nutrient agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.
(1)Prepare a flask of NB liquid media or two NA agar plates.
(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 18-24 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
|viability:||good viability, in 20h NB bacterial fluid is turbid, NA plate colony is obvious|
size: moderate color: orange-white shape: round edge: neat edge
wet and dry: wet and smooth: smooth and bright transparency: translucent uplift: middle bulge
|conclusion:||good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified|