Cordyceps militaris|BNCC143211 |BNCC

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Cordyceps militaris

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	37 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, obviously visible on integrated PDA agar medium, colonies are white, dry, vigorous, and spread The entire surface of the medium does not produce spores.
application	medicinal
Sharing mode	Public welfare sharing

Cordyceps militaris

No.: 143211

Storage conditions: 2~8 ℃

Product format : agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, integrated PDA. Comprehensive PDA: potato boiling juice 1000mL, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, glucose 20.0g, vitamin B1 10mg, agar 20g,pH natural. 121 ℃,15min. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

Recovery steps:

(1) Prepare 1 solid agar plate;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

⑤ Put the plates under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

test items	test result
viability:	good viability,in 7 days plate colony is obvious
colony morphology:	small filamentous fungi are obviously visible on integrated PDA agar medium, The colony is white, dry and vigorous, spreading to the entire surface of the culture medium without spores.
conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified