Enterobacter aerigens nucleic acid detection kit (fluorescent PCR method)|BNCC361454 |BNCC

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Klebsiella aerogenes nucleic acid detection kit (QPCR)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Experimental preparation: before the experiment, the sample is cultured with reference to GB 4789.41-2016; the various components in the kit are dissolved on ice before use, slightly mixed and centrifuged for a short time, and then placed on ice for standby; Reagent preparation: experimental reaction system (25μL),qPCR mixed reaction solution 15.3 μL, template 1 μL, sterile ddH2O8.7μL Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 57.4 ℃ 30s 40cycle; Fluorescence Channel: VIC.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	1
Separation substrate	Sputum, South Carolina Dept. of Health and Environmental Control
application	Mainly used for the detection of faeces in water samples, suspected contaminated water, food and other samples of Enterobacteriaceae
Sharing mode	Public welfare sharing