CHO host cell DNA residue detection kit (fluorescence PCR method)|BNCC370235 |BNCC

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CHO nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	1. Sample treatment: nucleic acid extraction shall be carried out according to the operation instructions of kit A. For details, please refer to Annex 1; 2. Detection experiment: dissolve the components in kit B on ice, mix well, centrifuge for a short time and put them on ice for later use; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Storage conditions	-20 ℃; protected from light
Safety level	1
application	Comply with Chinese Pharmacopoeia (2020 Edition), suitable for CHO host cell DNA residue detection
Sharing mode	Public welfare sharing