E. coli phage T1 quantitative standard control|BNCC362950 |BNCC

BeNa Culture Collection

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  • Escherichia coli bacteriophage T1 standard quality control product-BNCC

Escherichia coli bacteriophage T1 standard quality control product

  • Price： Contact
  • number：BNCC362950
  • Packing：Syringa bottle;(6.5±1.2)× 10 ^ 9PFU/bottle
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Escherichia coli bacteriophage T1 standard quality control product
Culture medium Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure Take 5mL of Escherichia coli phage T1 stock solution after filtration and sterilization, add 20mL(1:4) of protective agent, fully mix well, put into 7mL vial, put into 1mL each vial, plug into sterile cotton at the mouth of the vial, and put into a refrigerator at -80 ℃ for preservation.
Growth conditions culture temperature 37 ℃;18-24 hours; aerobic;
Storage conditions -20 ℃
Safety level 1
application This E. coli phage T1 quantitative standard quality control is collected by culturing E. coli host bacteria and phage T1. The titer and lysis efficiency of the collected phage were determined by double-layer agar counting method, which was mainly used for the quality control of phage PhiX174 positive.
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