Stbl3 (containing pLVX-AcGFP1-N1 plasmid) E. coli|359010 |BNCC

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BNCC > Plasmid > Stbl3 (containing pLVX-AcGFP1-N1 plasmid) E. coli
  • Escherichia coli Stbl3(pLVX-AcGFP1-N1)-BNCC

Escherichia coli Stbl3(pLVX-AcGFP1-N1)

  • Price： $ 186
  • number：BNCC359010
  • Packing：Freeze dried pellet
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Escherichia coli Stbl3(pLVX-AcGFP1-N1)
Culture medium BNCC362905
Description LB Medium with 100μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic
Subculture procedure ① Prepare one vial of sterile water and two 90mm resistance plates; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution, Agar plate onto antibiotic-containing agar plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Streak plates and pick single colonies onto 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment;
Storage conditions 2-8°C
Sharing mode Public welfare sharing
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