BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
Growth conditions | 30 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
morphology | Purity: pure |
application | Biological indicators of hot air disinfection control; L-desoxynojirimycin generation |
Sharing mode | Public welfare sharing |
Bacillus atrophaeus
Strain number: 356043
Name: Bacillus atrophaeus
Product format: quantitative bacteria tablets Number of viable bacteria: (3.0± 1.0)×10^6CFU/bottle
Product batch: 220418 Valid until: 2024.04.17
Inspection date: 2022.04.18 Sampling ratio: ≥2%
Report number: RS22356043-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet
project | Inspection description | results |
stain microscopy | gram negative and positive, cell morphology observed under microscope | G-bacilli |
colony morphology | observation of single colony morphology | Yellow, raised, bright, moist |
Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
Reference value of live bacteria | Non-selective medium plate, viable count | 3.0×10^6CFU/bottle |
Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | After the BNCC quality control inspection system quality inspection, the product is qualified |
Product list:1 bottle of (3.0± 1.0)×10^6CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;
Shelf life: unopened, below -20 ℃, 24 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. After full dissolution, it is (3.0± 1.0)×10^6CFU/mL, can be absorbed in proportion according to the test requirements;
4. Please use up immediately after opening, and do not store it;
Notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
(2) Used bacteria-carrying consumables, please autoclave and discard them.