Escherichia coli 

Storage conditions : -20 ℃

Validity period: 24 months

No. : 352027

Product format : freeze dried, 200ul

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Use : pharmacopoeia, GB 4789, control quality control strain & nbsp;

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used.  Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium 1: nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production.

Growth conditions  Temperature: 37 ℃  Gas environment: aerobic

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;  
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly; 
(4)Distribute it well in 200ml/plate; the plates are not allowed to seal by wrapping.
(5)If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And  the strains can be used when they grow

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