BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; microaerophilic (3%-5% O₂, 10% CO₂); |
| Subculture procedure | ① Thaw Frozen vial in water bath (30°C), shake vigorously to dissolve; ② Disinfect vial exterior with alcohol swab, then transfer to biosafety cabinet; ③ Unscrew cap, aspirate entire thawed solution, inoculate 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under the specified conditions. |
| Storage conditions | -80°C |
| Safety level | 2 |
| morphology | Pure |
| Sharing mode | Public welfare sharing |
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