Aspergillus oryzae|192716 |BNCC

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  • Aspergillus candidus-BNCC

Aspergillus candidus

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  • number：192716
  • Form：
    Small filamentous fungus. Colony formation is distinct on Chalky Agar medium. The Agar plate exhibits white, fluffy mycelium spreading across the surface. Later stages produce beige spores, with the reverse side of the medium turning pale yellow.
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Aspergillus candidus
Subculture procedure ① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions 28 ℃; 5-7 days; aerobic
Storage conditions 2-8 ℃
Safety level 1
morphology Small filamentous fungus. Colony formation is distinct on Chalky Agar medium. The Agar plate exhibits white, fluffy mycelium spreading across the surface. Later stages produce beige spores, with the reverse side of the medium turning pale yellow.
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1.Description 

1. Name: Aspergillus Candidus

2. BNCC No.:192716

3. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions 

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural, 121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature:  28-30 ℃,5-7 days

4.Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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