Green wood fungi (lignin wood fungi)|336558 |BNCC

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Trichoderma viride

Subculture procedure	Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, producing a large number of spores, producing green spores with irregular edges, white mycelium, rough surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Trichoderma viride

storage conditions: 2~8 ℃

number: 336558

product format: freeze dried, 200ul

shelf life: 24 months

Safety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 2 pieces of PDA plates or 2 agar slants;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; or transfer appropriate solution to

the agar slant.

(4)place the plates uprightly and agar slant obliquely under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability,in 5 days, plate colony is obvious
colony morphology:	The morphology of the bacteria is obviously visible in the integrated PDA agar medium, and the colony is white cotton wool at the initial stage, Later, green spores are produced, dry, wrinkled, and the reverse side of the colony is light yellowish brown, spreading on the surface of the flat plate.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the following figure, qualified

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