Bacillus amyloliquefaciens|BNCC191753 |BNCC

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Bacillus amyloliquefaciens

Culture medium	3.5% sodium chloride agar medium (NA-2): beef paste 3g, peptone 10.0g,NaCl 35.0g, agar 20.0g (not included in liquid medium),pH 7.2~7.4. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 2-3mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white bulge: middle bulge surface: bright and viscous texture: moist and easy to stir up
Separation substrate	Billed ephedra
application	Inhibition of Salmonella, Pear Green Mould Pathogen, Pear Black Mould Pathogen, and Anthrax Pathogen
Sharing mode	Public welfare sharing

Description

1. Name: Penicillium viridiatum

2. BNCC No.: 192006

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1. Comprehensive PDA agar: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.