BeNa Culture Collection
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| Culture medium | BNCC382470 |
| Description | Tryptic Soy Blood Agar with Hemin and Vitamin K₁ (TSA-HK) |
| Composition | Tryptone:15.0g, Phytone:5.0g, NaCl:5.0g, Sterile defibrinated sheep blood:5%, Agar:15.0g, Hemin:5mg, Menadione(Vitamin K3):0.5mg, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 3-4 days; anaerobic; |
| Subculture procedure | ① Prepare 1-2 fresh plates mentioned above (placed in anaerobic environment for deoxygenation for 24 hours in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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