Molluscaceae|354474 |BNCC

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Lachnospiraceae bacterium

Subculture procedure	① Prepare 1-2 plates as specified above (pre-conditioned in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner. Immediately add sterile water to break the seal, then use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water (pre-conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Growth conditions	37 ℃; 48-72 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli).
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Lachnospiraceae bacterium

Storage conditions: 2~8 ℃

No. 354474

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),4-5 days.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates(put in an anaerobic environment for deoxygenation in advance for 24 hours);
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 4days strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm shape: round edge: irregular transparency: opaque color: off-white bulge: middle convex surface: rough texture: easy to pick
Conclusion:	good viability, no abnormal colony morphology, qualified

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