Pseudomonas aeruginosa|357819 |BNCC

Essential Information Certificate Related Products

Roseateles saccharophilus

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	30 ℃; 48-72h; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Pelomonas saccharophila

Storage conditions : 2~8 ℃

No. : 357819

Product format: freeze dried,200ul

Validity period : 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30℃, aerobic, R2A agar medium, 2-3 days, R2A agar medium: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium hydrogen sulfate 0.3g , magnesium sulfate 0.1g, sodium pyruvate 0.3, agar 12 peptone 0.25 glucose 0.5g, pH7.2±0.2. Sterilize at 121°C for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 2 days strain layer become obvious, colony is typical
colony morphology (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: yellow uplift: middle raised surface: bright and smooth texture: moist and easy to stir
conclusion	good viability, no abnormal colony morphology, qualified

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