BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360798 |
| Description | Colombia Agar Chocolate Plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃) |
| Growth conditions | 30 ℃; 3-5 days; aerobic; |
| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 2-4mm, circular, with a gray white front, flat shape, smooth surface, bright surface, moist texture, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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