DH10Bac Escherichia coli|353824 |BNCC

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Escherichia coli DH10Bac

Subculture procedure	1. Prepare one sterile liquid culture tube (containing 5–10 mL of medium) and two 90 mm agar plates. 2. After disinfecting the outer wall of the vial, open the aluminum cap and rubber stopper under aseptic conditions. Aspirate approximately 0.5 mL of liquid medium and inject it into the vial, gently pipetting to dissolve the bacterial pellet. 3. Once the pellet is fully dissolved, aspirate the entire suspension and transfer it back into the liquid culture tube, mixing thoroughly. 4. From the liquid culture tube, aspirate the mixed suspension and spread it onto the agar plates (200 μL per Agar plate). 5. Place both the tube and the plates under the specified incubation conditions. Do not seal or wrap the plates. After 18–24 hours, observe for visible turbidity in the liquid culture and colony/lawn growth on the plates. 6. If no growth is observed, continue incubation up to 72 hours. Once growth is confirmed, the culture can be used according to experimental requirements.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	1
morphology	Growth on the streak Agar plate.
Sharing mode	Public welfare sharing

Escherichia coli DH10Bac

No. : 353824

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 1

Validity period: 5 years

Use : Used for gene cloning (with Kan/Tet resistance)

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Medium :LB agar medium yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, agar 15.0g, distilled water 1.0 L,pH 7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6) If it does not grow in 18-24 hours, culture is continued until 72 hours is reached. And the strains can be used when they grow

Growth conditions Temperature: 37 ℃ Gas environment: Aerobic time: 18-24h

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