BeNa Culture Collection
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| Growth conditions | 37°C; 2-3 days; Microaerophilic (3%-5% O₂, 10% CO₂); |
| Subculture procedure | Remove one vial of frozen ureaplasma stock (500 μL) and six tubes pre-filled with medium (one as control; 5 for culture). Add all thawed ureaplasma to the first tube (containing 4.5 mL prepared ureaplasma medium), shake thoroughly, then transfer 0.5 mL to the next culture tube. Repeat to create 5 dilution gradients. Incubate all 5 diluted tubes and 1 control tube at 37°C under aerobic conditions for 18 hours. |
| Storage conditions | liquid nitrogen |
| Sharing mode | Public welfare sharing |
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