Dengue virus 3|390326 |BNCC

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Dengue virus type 3

Growth conditions	37°C; 5% CO₂ + 95% air;
Subculture procedure	① Inoculation procedure: Seed host cells into T25 flasks 24–48 hours prior to inoculation, and prepare for viral inoculation when the host cell density reaches 80–90%; Remove the cell culture medium and inoculate 100–500 μL of viral suspension. Allow adsorption for 1–2 hours under culture conditions, gently shaking the T25 flask every 15–30 minutes to ensure even viral distribution; add 6 mL of viral growth medium to complete adsorption, transfer to the incubator for continued culture, and monitor cytopathic effects periodically; ② Virus Harvesting: Once all host cells exhibit cytopathic effects, begin harvesting the viral supernatant. During harvesting, freeze-thaw the cells 1–2 times, then filter through a 0.22 μm pore size filter; the collected filtrate constitutes the viral supernatant.
Storage conditions	Liquid nitrogen
Sharing mode	Public welfare sharing