BeNa Culture Collection
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| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1 vial of sterile water and 2 x 90mm resistant plates; ② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve completely, then spread solution onto antibiotic plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Streak plates and pick single colonies onto 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment; |
| Storage conditions | 2-8°C |
| Sharing mode | Public welfare sharing |
Mycobacterium tuberculosis nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium tuberculosis nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395935
Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur] nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur] nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395936
Mycobacterium bovis Karlson and Lessel nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium bovis Karlson and Lessel nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395937
Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive)(Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398890
Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive)(Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC399115
Enterococcus faecalis nucleic acid reference (Heat inactivated) (Strongly positive)(Enterococcus faecalis nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC400124