DH5α (recombinant plasmid containing Vibrio cholerae ompW gene) E. coli|360421 |BNCC

Essential Information Certificate Related Products

Escherichia coli DH5α（Plasmid vibrio choleraelompW gene）

Subculture procedure	① Thaw Frozen vials in a water bath (37°C), shaking vigorously to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under the specified conditions.
Growth conditions	37°C; 18-24h; aerobic
Storage conditions	-80°C
Safety level	1
Sharing mode	Public welfare sharing

water quality fecal coliform negative quality control sample certificate

1. Product information:

Sample name: water quality fecal coliform negative quality control sample (multi-tube fermentation method)

Sample number: BNCC 360421

sample batch: 211203

2. Product features:

description	appearance is white powder (freeze-dried powder), containing protective agent, 7mL penile bottle packaging
traceability	Enterobacter aerogenes ATCC13048
concentration value	300~3000MPN/L
reference value	2200MPN/L
uniformity and stability	Random sampling, good uniformity of results; Long-term stability test, stability qualified
applicable method	"HJ 347.2-2018 water quality determination of fecal coliform multi-tube fermentation method"
* Note: If you have any questions about quality control samples, please contact BNCC for help

3.Instructions

Dissolve the material by 100mL of sterile water or sterile saline. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3-4ml of sterile water is immediately added, dissolved and transferred into a sterile bottle; rinse the inner wall of the penicillin bottle with the remaining sterile water, and transfer all the solution to the former sterile bottle. That is positive quality control material，totaling 500ml, with a concentration of 300~3000MPN/L。

4. Precautions

1. Upon receipt, please keep it properly. if it is damaged, please contact us within 24 hours.

2. Please handle in a biosafety cabinet under sterile conditions. The material shall be used up once opened.

3. the product contains risk-3 pathogenic organisms，please do not directly touch the samples with your hands, please discard the residual samples after high temperature and high pressure sterilization.

5. Preservation and transportation

long-term storage: -20 ℃, valid for 1 year; ship on ice bag; the shipping time should not exceed 10 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn tel: 400-6699-833

Download certificate