DH5α (containing pETBlue-2 plasmid) E. coli|353856 |BNCC

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Escherichia coli DH5α(pETBlue-2)

Culture medium	BNCC361029
Description	LB Medium with 50μg/mL Ampicillin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	1. Prepare one sterile liquid test tube (containing 5-10mL medium) and two 90mm plates; 2. After disinfecting the vial wall, open the aluminum cap and rubber stopper under sterile conditions. Pipette approximately 0.5 mL of liquid medium into the vial and gently tap to dissolve the bacterial pellet; 3. Once the pellet is fully dissolved, aspirate the entire solution back into the liquid test tube and mix thoroughly; 4. Pipette the mixture from the test tube onto plates, 200μL per well; 5. Incubate all test tubes and plates under the specified conditions. Do not seal or wrap plates. Observe for visible turbidity in the culture medium and colony or biofilm growth on plates after 18–24 hours; 6. If no growth is observed, continue incubation for up to 72 hours. Use the culture as required for the experiment.
Storage conditions	-20°C
Sharing mode	Public welfare sharing