BeNa Culture Collection
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| Subculture procedure | ① Prepare 1 sterile water tube and 2 90mm resistant plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μ L per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 ℃ on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction; |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| Sharing mode | Public welfare sharing |
Biochemical ldentification Kit for Klebsiella pneumoniae
BNCC361613
Water quality fecal coliform standard quality control sample (Membrane membrane method)
BNCC360398
Serwater fecal coliform positive control quality control sample (Multi-tube fermentation method)
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Standard quality control sample of clostridium perfringens in drinking water
BNCC362541
Shigella quality control sample of sewage from medical institutions
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Quantitative strains of Micrococcus sp.
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