BL21 (DE3) (containing pET-32a (+) - NP7 plasmid) Escherichia coli|354652 |BNCC

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Escherichia coli BL21(DE3)(pET-32a(+)-NSP7)

Subculture procedure	① Dissolve the Frozen vial tube in a water bath (37 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing