BL21 (DE3) (containing pET-32a (+) - NSP16 plasmid) Escherichia coli|354658 |BNCC

BeNa Culture Collection

  • Escherichia coli BL21(DE3)(pET-32a(+)-NSP16)-BNCC

Escherichia coli BL21(DE3)(pET-32a(+)-NSP16)

  • Price: $ 186
  • number:BNCC354658
  • Packing:Frozen vial tube
Essential Information Related Products
Escherichia coli BL21(DE3)(pET-32a(+)-NSP16)
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37 ℃; 18-24h; aerobic;
Subculture procedure ① Dissolve the Frozen vial tube in a water bath (37 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions -80 ℃
Sharing mode Public welfare sharing
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