DH5α (containing pcDNA3.1-ERBB-2 plasmid) E. coli|358094 |BNCC

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Escherichia coli DH5α(pcDNA3.1-ERBB-2)

Subculture procedure	① Thaw cryovials in a water bath (37°C), shaking vigorously to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and plate onto 1-2 agar plates (approx. 200μL per plate); ④ Spread evenly and incubate under the specified conditions.
Growth conditions	37°C; 18-24h; aerobic;
Storage conditions	-80°C
Safety level	1
Sharing mode	Public welfare sharing