DH5 α (containing pRS426 phage in E. coli plasmid) Escherichia coli|358122 |BNCC

Essential Information Related Products

Escherichia coli DH5α(pRS426)

Subculture procedure	① Dissolve the cryovial tube in a water bath (37 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing