DH5 α (containing pRS426 phage in E. coli plasmid) Escherichia coli|358122 |BNCC

BeNa Culture Collection

  • Escherichia coli DH5α(pRS426)-BNCC

Escherichia coli DH5α(pRS426)

  • Price: $ 186
  • number:BNCC358122
  • Packing:Cryovial tube
Essential Information Related Products
Escherichia coli DH5α(pRS426)
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37 ℃; 18-24h; aerobic;
Subculture procedure ① Dissolve the cryovial tube in a water bath (37 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions -80 ℃
Sharing mode Public welfare sharing
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