BL21 (DE3) (containing pET-32a (+) - S-Protein RBD) Escherichia coli|354686 |BNCC

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Escherichia coli BL21(DE3)(pET-32a(+)-S-protein RBD)

Subculture procedure	1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μ L per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing