BL21 (DE3) (containing pET-32a (+) - S-Protein RBD) Escherichia coli|354686 |BNCC

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Escherichia coli BL21(DE3)(pET-32a(+)-S-protein RBD)

Culture medium	BNCC361029
Description	LB Medium with 50μg/mL Ampicillin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μ L per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements.
Storage conditions	-80 ℃
Sharing mode	Public welfare sharing