DH5α (containing pBiT1.1-N [TK-LgBiT] plasmid) E. coli|359211 |BNCC

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DH5α (containing pBiT1.1-N [TK-LgBiT] plasmid) E. coli-BNCC
DH5α (containing pBiT1.1-N [TK-LgBiT] plasmid) E. coli-BNCC
  • Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])-BNCC
  • Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])-BNCC
  • Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])-BNCC

Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])

  • Price： $ 215
  • number：BNCC359211
  • Packing：Freeze dried pellet
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Essential Information Related Products
Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])
Growth conditions 37°C; 18-24h; aerobic;
Subculture procedure ① Prepare 1 vial of sterile water and 2 90mm resistant plates; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve completely, then spread solution onto antibiotic plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50 mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Storage conditions 2-8°C
Sharing mode Public welfare sharing
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