DH5α (containing pEGFP-LC3B-r plasmid) E. coli|358943 |BNCC

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BNCC > Plasmid > DH5α (containing pEGFP-LC3B-r plasmid) E. coli
  • Escherichia coli DH5α(pEGFP-LC3B-r)-BNCC

Escherichia coli DH5α(pEGFP-LC3B-r)

  • Price： $ 186
  • number：BNCC358943
  • Packing：Frozen vial
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Essential Information Related Products
Escherichia coli DH5α(pEGFP-LC3B-r)
Culture medium BNCC361023
Description LB Medium with 50μg/mL Kanamycin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic
Subculture procedure ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions -80°C
Sharing mode Public welfare sharing
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