DH5α (containing PVAX1 plasmid) E. coli|358946 |BNCC

BeNa Culture Collection

  • Escherichia coli DH5α(PVAX1)-BNCC

Escherichia coli DH5α(PVAX1)

  • Price: $ 186
  • number:BNCC358946
  • Packing:Frozen vial
Essential Information Related Products
Escherichia coli DH5α(PVAX1)
Culture medium BNCC361023
Description LB Medium with 50μg/mL Kanamycin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic
Subculture procedure ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into the lyophilized tube. After complete dissolution, return it to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions -80°C
Sharing mode Public welfare sharing
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