BeNa Culture Collection
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| Subculture procedure | ① Prepare one vial of sterile water and two 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol burner, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube, dissolve completely, then Agar plate onto antibiotic plates (200μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment; |
| Growth conditions | 37°C; 18-24h; aerobic |
| Storage conditions | -80°C |
| Safety level | 1 |
| Sharing mode | Public welfare sharing |
Seawater total bacterial colony count quality control sample
BNCC369590
Standard quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method)
BNCC360414
Water quality fecal coliform positive quality control samples (Paper Rapid Method)
BNCC360408
Quantitative strains of escherichia coli
BNCC364778
Reference material of total bacterial count in food
BNCC359084
Quantitative strains of bacillus subtilis subsp. spizizenii Nakamura et al.
BNCC353859
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