DH5α (containing pUF1-dCas9-Sir2a plasmid) E. coli|365230 |BNCC

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Escherichia coli DH5α（pUF1-dCas9-Sir2a）

Subculture procedure	① Prepare 1 vial of sterile water and 2 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, Agar plate onto antibiotic-resistant plates (200μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment;
Growth conditions	37°C; 18-24h; aerobic;
Storage conditions	-80°C
Safety level	1
Sharing mode	Public welfare sharing