DH5α (containing pcDNA-Ikkb-FLAG WT plasmid) E. coli|365221 |BNCC

BeNa Culture Collection

BNCC > Plasmid > DH5α (containing pcDNA-Ikkb-FLAG WT plasmid) E. coli
  • Escherichia coli DH5α（pcDNA-Ikkb-FLAG WT ）-BNCC

Escherichia coli DH5α（pcDNA-Ikkb-FLAG WT ）

  • Price： $ 186
  • number：BNCC365221
  • Packing：Frozen vial
Add to cart
View Cart
Essential Information Related Products
Escherichia coli DH5α（pcDNA-Ikkb-FLAG WT ）
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic;
Subculture procedure ① Prepare one vial of sterile water and two 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution, spread solution onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Storage conditions -80°C
Sharing mode Public welfare sharing
Related Products
Popular Products
Please set your password: