DH5α (containing pKK232-8 E. coli pKK series expression plasmid)|365207 |BNCC

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BNCC > Plasmid > DH5α (containing pKK232-8 E. coli pKK series expression plasmid)
  • Escherichia coli DH5α（pKK232-8）-BNCC

Escherichia coli DH5α（pKK232-8）

  • Price： $ 186
  • number：BNCC365207
  • Packing：Frozen vial
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Essential Information Related Products
Escherichia coli DH5α（pKK232-8）
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions E. coli
Subculture procedure ① Prepare one vial of sterile water and two 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution, spread solution onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50 mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Storage conditions -80℃
Sharing mode Public welfare sharing
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