BeNa Culture Collection
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| Subculture procedure | ① Prepare one vial of sterile water and two 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized vial, dissolve completely, then Agar plate onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment; |
| Growth conditions | 37°C; 18-24h; aerobic |
| Storage conditions | -80°C |
| Safety level | 1 |
| Sharing mode | Public welfare sharing |
Standard quality control samples of heat-resistant coliform bacteria in drinking water (multi-tube fermentation method)
BNCC360414
Water quality fecal coliform standard quality control sample (Paper Rapid Method)
BNCC360406
Quantitative strains of pseudomonas aeruginosa
BNCC365156
Quality control sample of total bacterial count in drinking water
BNCC381093
Fecal coliform positive control quality control sample (Membrane membrane method)
BNCC359576
Reference material of staphylococcus aureus count in food
BNCC359095
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