DH5α (containing pET28a-Cas9-Cys plasmid) E. coli|365179 |BNCC

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Escherichia coli DH5α（pET28a-Cas9-Cys ）

Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare 1 vial of sterile water and 2 90mm resistant plates;② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule using forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution, spread solution onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Storage conditions	-80°C
Sharing mode	Public welfare sharing