DH5α E. coli (containing pYD1 plasmid)|359036 |BNCC

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BNCC > Plasmid > DH5α E. coli (containing pYD1 plasmid)
  • Escherichia coli DH5α(pYD1)-BNCC

Escherichia coli DH5α(pYD1)

  • Price： $ 186
  • number：BNCC359036
  • Packing：Freeze dried pellet
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Escherichia coli DH5α(pYD1)
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic;
Subculture procedure ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions -80°C
Sharing mode Public welfare sharing
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