DH5α (containing pCBASceI plasmid) E. coli|359697 |BNCC

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BNCC > Plasmid > DH5α (containing pCBASceI plasmid) E. coli
  • Escherichia coli DH5α(pCBASceI)-BNCC

Escherichia coli DH5α(pCBASceI)

  • Price： $ 186
  • number：BNCC359697
  • Packing：Freeze dried pellet
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Essential Information Related Products
Escherichia coli DH5α(pCBASceI)
Culture medium BNCC361029
Description LB Medium with 50μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic;
Subculture procedure ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5 mL of liquid medium into the lyophilized tube. After complete dissolution, transfer it back to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions 2-8°C
Sharing mode Public welfare sharing
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