DH5α (containing pEnCMV-KRAS WT-3×FLAG plasmid) E. coli|359699 |BNCC

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Escherichia coli DH5α(pEnCMV-KRAS WT-3×FLAG)

Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare one tube containing 5-10mL liquid medium and two plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	-80°C
Sharing mode	Public welfare sharing