Stbl3 (containing pBI121 plasmid) E. coli|359027 |BNCC

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  • Escherichia coli Stbl3(pBI121)-BNCC

Escherichia coli Stbl3(pBI121)

  • Price： $ 186
  • number：BNCC359027
  • Packing：Frozen vial
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Essential Information Related Products
Escherichia coli Stbl3(pBI121)
Culture medium BNCC361023
Description LB Medium with 50μg/mL Kanamycin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic
Subculture procedure ① Prepare one test tube containing 5-10mL liquid medium and two agar plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into the lyophilized tube. After complete dissolution, return it to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions -80°C
Sharing mode Public welfare sharing
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