BeNa Culture Collection
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| Growth conditions | 37°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1 tube containing 5-10 mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the lyophilized tube, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
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