Stbl3 (containing Cyto-roGFP plasmid) E. coli|359018 |BNCC

BeNa Culture Collection

BNCC > Plasmid > Stbl3 (containing Cyto-roGFP plasmid) E. coli
  • Escherichia coli Stbl3(Cyto-roGFP)-BNCC

Escherichia coli Stbl3(Cyto-roGFP)

  • Price： $ 186
  • number：BNCC359018
  • Packing：Frozen vial
Add to cart
View Cart
Essential Information Related Products
Escherichia coli Stbl3(Cyto-roGFP)
Culture medium BNCC362905
Description LB Medium with 100μg/mL Ampicillin
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions 37°C; 18-24h; aerobic;
Subculture procedure ① Prepare 1 vial of sterile water and 2 x 90mm resistant plates; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve completely, then Agar plate onto antibiotic plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Streak plates and pick single colonies onto 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment;
Storage conditions 2-8°C
Sharing mode Public welfare sharing
Related Products
Popular Products
Please set your password: