DH5 α (containing pNICKclos2.0 plasmid) Escherichia coli|365204 |BNCC

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Escherichia coli DH5α（PESC-HIS）

Subculture procedure	① Dissolve the Frozen vial tube in a water bath (37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing