DH5 α (containing pUC18 plasmid) Escherichia coli|353825 |BNCC

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Escherichia coli DH5α(pUC18)

Subculture procedure	① Prepare 1 sterile water and 2 90mm resistant tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μ L per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 ℃ on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction;
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
Sharing mode	Public welfare sharing

Escherichia coli DH5α( pUC18)

No.: 353825

Product form: tablets in penicillin bottle

Storage conditions: -20 ℃

Biosafety level: 1

Validity period: 5 years

Usage: pUC18 plasmid extraction

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Medium:LB resistant agar medium yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, agar 15.0g, distilled water 1.0 L,pH 7.0. Sterilization at 121 ℃ for 15min. After sterilization, ampicillin sodium is added after cooling down, so that the final content of ampicillin sodium in the culture medium is 50ug/mL.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)Sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6) If it does not grow, culture is continued until 72 hours is reached. And the strains can be used when they grow

culture conditions

temperature: 37 ℃ Gas environment: aerobic Time: 18-24h

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