BeNa Culture Collection
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| Growth conditions | 28 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 1 sterile water tube and 2 90mm resistant plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μ L per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 ℃ on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction; |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
Mycobacterium tuberculosis nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium tuberculosis nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395935
Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur] nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur] nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395936
Mycobacterium bovis Karlson and Lessel nucleic acid reference(Heat inactivated) (Strongly positive)(Mycobacterium bovis Karlson and Lessel nucleic acid reference(Heat inactivated) (Strongly positive))
BNCC395937
Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive)(Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398890
Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive)(Corynebacterium diphtheriae nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC399115
Enterococcus faecalis nucleic acid reference (Heat inactivated) (Strongly positive)(Enterococcus faecalis nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC400124