Tawara Nodamura virus (PvNV) plasmid DNA|397424 |BNCC

Essential Information Related Products

Plasmid DNA of Penaeus Vannamei Nodavirus (PvNV)

Growth conditions	37 ℃; 18-24 days; Aerobic;
Subculture procedure	① Dissolve: Add 20 μ L of sterile water to the plasmid powder and let it stand at Room Temperature for 1 minute; ② Mixing: Mix 200 μL of competent cells with 5-10 μ l of plasmid DNA and place on ice for 30 minutes; ③ Heat shock introduction: let stand at 42 ℃ for 90 seconds; ④ Shrinkage film pores: ice bath for 2 minutes; ⑤ Repair culture: Add 800 μ l of liquid culture medium to each tube and culture on a shaker at 37 ℃ for 1 hour (150 r/min); ⑥ Screening and cultivation: Apply an appropriate volume (100 μ l) of revived cells onto corresponding resistant LB plates, place them upright in a petri dish for 30 minutes (the agar surface must be dry), invert and culture for 12-16 hours, and colonies will appear; ⑦ Extraction: Select monoclonal colonies into the corresponding resistant LB liquid medium, shake and culture for 12-16 hours, and extract plasmids according to experimental needs. Basic medium (LB): yeast extract 5.0g, peptone 10.0g, NaCl 10.0g, distilled water 1.0L, pH 7.0. Sterilize at 121 ℃ for 15 minutes. (When the culture medium is sterilized and cooled to 48 ℃, add the antibiotic to achieve a final content of 50ng/mL.)
Storage conditions	2-8 ℃
Sharing mode	Public welfare sharing