BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361029 |
| Description | LB Medium with 50μg/mL Ampicillin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
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