Aspergillus fumigatus|187486 |BNCC

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Aspergillus fumigatus

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it apart with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense approximately 200μL onto each of two plates; spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once the fungal strain grows.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungi produce distinct colonies on PDA medium. Initial mycelium appears white, dense, and vigorous, later producing gray spores that cover the entire Agar plate.
Sharing mode	Public welfare sharing

Aspergillus fumigatus

BNCC No.:187486

Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1. 5 °Bé wort agar: 5 °Bé wort1.0L, agar 15.0g, naturally pH. 121℃,15min.

2. Atmosphere: aerobic

3. Temperature: 28-30℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterili

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