BeNa Culture Collection
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| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Dissolve the frozen storage tube in a 37 ℃ water bath, shake quickly to dissolve, wipe the outer wall of the frozen storage tube with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, pour it into 1-2 agar plates, and apply evenly; ④ Cover the agar surface with sufficient (1-4ml) tryptone soy broth medium to maintain a liquid film throughout the entire cultivation period, and prevent the culture from growing on dry surfaces. ⑤ Place the agar plate under the above cultivation conditions for cultivation. |
| Growth conditions | 37 ℃; 3-4 days; 5% CO2 |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, semi transparent, dark color, protruding in the middle, smooth surface, moist texture, and does not produce pigments, G- |
| Sharing mode | Public welfare sharing |
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